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KMID : 0380620130450010120
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Korean Journal of Food Science and Technology
2013 Volume.45 No. 1 p.120 ~ p.125
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Biocontrol of Biofilm-forming Bacillus cereus by Using Organic Acid, Ethanol, and Sodium Chloride
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Lee Young-Duck
Yoo Hye-Lim
Park Jong-Hyun
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Abstract
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Food poisoning by Bacillus cereus is one of the common food-borne diseases and B. cereus is widely
distributed in natural and commercial products owing to the strong resistance caused by biofilm or spore. The ethanol,
NaCl, and organic acids of acetic acid, citric acid, and lactic acid for biocontrol of biofilm-forming B. cereus on glass
wool were investigated. The biofilm on glass wool was observed in many developments after 48 h incubation. As the
results of reduction of biofilm-forming B. cereus by sanitizers, reduction levels of each organic acid treatment ranged to
5-6 log CFU/g-glass wool. In case of combination treatments of 20% ethanol, 10% NaCl, and 1% of each organic acid for
1-5 min, the reduction level of biofilm-forming B. cereus was 7-8 log CFU/g-glass wool. Therefore, combination
treatments of ethanol, NaCl, and an organic acid might effectively reduce biofilm-forming B. cereus in various food
processes and industries.
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KEYWORD
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biofilm, Bacillus cereus, ethanol, NaCl, organic acid
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